logrank kaplan-meier and gehan-breslow-wilcoxen survival tests  (GraphPad Software Inc)

Journal: Frontiers in Microbiology

Article Title: Therapeutic efficacy of n -Docosanol against velogenic Newcastle disease virus infection in domestic chickens

doi: 10.3389/fmicb.2022.1049037

Figure Lengend Snippet: The survival rate of experimentally NDV infected chickens during and after treatment with 20, 40, and 60 mg/k.g BW n -docosanol. The number of alive chickens in each group was recorded at 1–15 days post the first n -docosanol dosage injection. The survival rate was graphed using GraphPad Prism version 6.01 and analyzed by Kaplan–Meier estimator with Logrank test. A significant difference ( value of p of <0.001) appeared between the untreated group and both the high (60 mg/kg) and medium (40 mg/kg) dosage groups.

Article Snippet: Kaplan–Meier estimator with Logrank test of GraphPad Prism version 6.01 was used to compare the numbers of survived chickens post-challenge.

Techniques: Infection, Injection

Journal: Breast Cancer Research : BCR

Article Title: Glucocorticoid receptors are required effectors of TGFβ1-induced p38 MAPK signaling to advanced cancer phenotypes in triple-negative breast cancer

doi: 10.1186/s13058-020-01277-8

Figure Lengend Snippet: 14-3-3ζ is required for TGFβ1-induced TNBC cell migration. a Expression of 14-3-3ζ in breast cancer patients was analyzed using the METABRIC database ( n = 1700). Significance was assessed by one-way ANOVA and Tukey post hoc for comparison between groups (****, p < 0.0001). b Survival analysis for patients from the METABRIC cohort and with mRNA microarray profiling for 14-3-3ζ were stratified into two groups: expression higher than median mRNA expression of 14-3-3ζ (upper 50th percentile) or lower than median (bottom 50th percentile). The results were analyzed for significance with a logrank p value of p < 0.0001 ( n = 1981). c Western blot analysis for 14-3-3ζ of different cell lines indicated. Densitometry values for the 14-3-3ζ levels are indicated relative to non-tumorigenic MCF10A cells. d Western blot analysis of 14-3-3ζ protein levels in MDA-MB-231 cells expressing either shcontrol or sh-14-3-3ζ; two pools of each respective shcontrol are shown. Densitometry values for the 14-3-3ζ levels are shown relative to shcontrol #1. Transwell migration assays were used to test the migratory activity of shcontrol and sh14-3-3ζ MDA-MB-231 cells. TGFβ1 (10 ng/mL) was used as the chemoattractant and cells were allowed to migrate for 18 h. The mean of three independent biological replicate experiments is shown ± SD. Statistical significance was assessed by two-way ANOVA and Tukey post hoc corrections (****, p < 0.0001). e Western blot analysis was performed on immunocomplexes and whole-cell lysates to evaluate the interaction of 14-3-3ζ with GR in response to Veh, TGFβ1 (10 ng/mL), Dex (1 μM), TGFβ1+Dex, and H 2 O 2 (100 μM; positive control for GR phosphorylation). Densitometry values for the 14-3-3ζ levels in the GR IP groups are shown relative to vehicle control. IgG was used as a negative control

Article Snippet: Kaplan-Meier plots and logrank test was used to assess the differences in overall survival between the cohorts using PRISM (GraphPad).

Techniques: Migration, Expressing, Comparison, Microarray, Western Blot, Activity Assay, Positive Control, Phospho-proteomics, Control, Negative Control

Journal: Breast Cancer Research : BCR

Article Title: Glucocorticoid receptors are required effectors of TGFβ1-induced p38 MAPK signaling to advanced cancer phenotypes in triple-negative breast cancer

doi: 10.1186/s13058-020-01277-8

Figure Lengend Snippet: P-S134-GR promotes the expression of a 24-gene signature that correlated with poor prognosis in BC. a Venn diagram showing genes that are upregulated by TGFβ1 in MDA-MB-231 cells expressing either wt-GR or S134A-GR. The cutoffs used to define upregulation were a Benjamini-Hochberg value of less than 0.05 and a log2 fold-change of at least 1.5. b Supervised clustering of the 39 genes significantly upregulated in wt-GR cells by TGFβ1. c LEFTY2 and PIK3IP1 mRNA levels were assessed by qRT-PCR following normalization to Actin. Wt-GR and S134A-GR MDA-MB-231 cells were treated with either vehicle or TGFβ1 (10 ng/mL) for 6 h. Mean expression of three independent replicates ± SD is shown. Statistical significance was assessed by two-way ANOVA and Tukey post hoc for comparison within groups (*, p < 0.05). d Either wt-GR or S134A-GR MDA-MB-231 cells were treated with either vehicle or TGFβ1 (10 ng/mL) for 1 h. ChIP assays for the LEFTY2 and PIK3IP1 promoters to evaluate recruitment of GR were performed. The mean of three independent biological replicates are shown ± SD. Statistical significance was assessed by two-way ANOVA and Tukey post hoc for comparison within groups (*, p < 0.05; ****, p < 0.0001). e Kaplan-Meier curves are shown for the METABRIC dataset ( n = 1904). Patients were separated by calculating the median expression of the previously identified GR Ser134-dependent 24-gene signature. This analysis was limited to 5 years of survival data. The results are significant with a logrank p value of p = .0008. f SurvExpress was used to stratify TCGA breast cancer cohort based on their median prognostic index as determined by SurvExpress with the TGFβ1 pS134-GR 24-gene signature. The results were analyzed for significance with a logrank p value of p = .0030

Article Snippet: Kaplan-Meier plots and logrank test was used to assess the differences in overall survival between the cohorts using PRISM (GraphPad).

Techniques: Expressing, Quantitative RT-PCR, Comparison